1.經(jīng)典細(xì)胞培養(yǎng)書籍下載culture of animal cells（10.7M）

Culture of Animal Cells: A Manual of Basic Technique, 5th Edition
R. Ian Freshney 
ISBN: 978-0-471-45329-1
Hardcover
672 pages
July 2005

The most complete resource on the techniques, equipment,principles, and practices of animal cell culture

Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced.

The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes:
* An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols
* New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering
* An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment
* Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text
* Fully updated references
* The clearest, most consistent presentation of step-by-step protocols available
* Numerous diagrams, photographs, tables, and charts
* Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites

Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. 


List of Figures. 
List of Color Plates. 

Preface. 

Abbreviations. 

1. Introduction. 

2. Training Programs. 

3. Biology of Cultured Cells. 

4. Laboratory Design and Layout. 

5. Equipment. 

6. Aseptic Technique. 

7. Safety, Bioethics, and Validation. 

8. Culture Vessels and Substrates. 

9. Defined Media and Supplements. 

10. Serum-Free Media. 

11. Preparation and Sterilization. 

12. Primary Culture. 

13. Subculture and Cell Lines. 

14. Cloning and Selection. 

15. Cell Separation. 

16. Characterization. 

17. Differentiation. 

18. Transformation and Immortalization. 

19. Contamination. 

20. Cryopreservation. 

21. Quantitation. 

22. Cytotoxocity. 

23. Culture of Specific Cell Types. 

24. Culture of Tumor Cells. 

25. Organotypic Culture. 

26. Scale-Up. 

27. Specialized Techniques. 

28. Problem Solving. 

29. In Conclusion. 

Appendix I: Preparation of Reagents. 

Appendix II: Sources of Equipment and Materials. 

Appendix III: Suppliers and Other Resources. 

Appendix IV: Glossary. 

Appendix V: General Textbooks and Relevant Journals. 



 

2.酵母遺傳學(xué)方法實(shí)驗(yàn)指南（2.4M）

3.白純化、免疫、信息學(xué)、細(xì)胞實(shí)驗(yàn)經(jīng)典實(shí)驗(yàn)匯總（5.4M）

主要內(nèi)容：

下游蛋白純化技術(shù)電子書：
Native page.exe；SDS-PAGE(SDS—聚丙烯酰胺凝膠電泳).exe；Western Blot(免疫印跡).exe；包涵體復(fù)性.exe；超濾.exe；蛋白質(zhì)濃縮及濃度測定.exe；等電聚焦凝膠電泳.exe；多肽人工合成.exe；高效液相.exe；離子交換層析.exe；凝膠過濾.exe；親和層析.exe；細(xì)胞破碎.exe；鹽析.exe

免疫學(xué)電子書：
ELISA.exe；分子診斷.exe；快速診斷.exe；免疫PCR(Immuno PCR,Im-PCR).exe；免疫組化技術(shù).exe；生物素-親和素系統(tǒng).exe
疫苗.exe

單抗、多抗及其制備電子書籍：
IgG的分離與提純.exe；IgY的應(yīng)用與研究進(jìn)展.exe；單克隆抗體.exe；抗體的純化.exe；抗體介紹.exe；佐劑的作用和制備.exe

生物信息學(xué)電子書籍：
表達(dá)序列標(biāo)簽（ESTs）及其應(yīng)用.exe；蛋白質(zhì)結(jié)構(gòu)預(yù)測.exe；分子進(jìn)化與系統(tǒng)進(jìn)化樹的構(gòu)建.exe；生物信息學(xué)概述.exe；生物信息學(xué)數(shù)據(jù)庫及查詢.exe；序列比對及數(shù)據(jù)庫搜索.exe；序列分析預(yù)測.exe

真核細(xì)胞表達(dá)電子書：
CHO細(xì)胞表達(dá)系統(tǒng).exe；酵母細(xì)胞表達(dá)系統(tǒng).exe；真核細(xì)胞表達(dá)系統(tǒng).exe

細(xì)胞培養(yǎng)及動(dòng)物飼養(yǎng)電子書籍
實(shí)驗(yàn)動(dòng)物飼養(yǎng).exe；細(xì)胞培養(yǎng).exe；細(xì)胞培養(yǎng)基.exe

4.蛋白純化手冊（1.55M）

5.蛋白純化、信息學(xué)、真核細(xì)胞表達(dá)實(shí)驗(yàn)技術(shù)大全

主要內(nèi)容：
下游蛋白純化技術(shù)電子書
Native page.exe；SDS-PAGE(SDS—聚丙烯酰胺凝膠電泳).exe；Western Blot(免疫印跡).exe；包涵體復(fù)性.exe；超濾.exe；蛋白質(zhì)濃縮及濃度測定.exe；等電聚焦凝膠電泳.exe；多肽人工合成.exe；高效液相.exe；離子交換層析.exe；凝膠過濾.exe；親和層析.exe；細(xì)胞破碎.exe；鹽析.exe

生物信息學(xué)電子書籍：
表達(dá)序列標(biāo)簽（ESTs）及其應(yīng)用.exe；蛋白質(zhì)結(jié)構(gòu)預(yù)測.exe；分子進(jìn)化與系統(tǒng)進(jìn)化樹的構(gòu)建.exe；生物信息學(xué)概述.exe；生物信息學(xué)數(shù)據(jù)庫及查詢.exe；序列比對及數(shù)據(jù)庫搜索.exe；序列分析預(yù)測.exe

真核細(xì)胞表達(dá)電子書：
CHO細(xì)胞表達(dá)系統(tǒng).exe；酵母細(xì)胞表達(dá)系統(tǒng).exe；真核細(xì)胞表達(dá)系統(tǒng).exe

細(xì)胞培養(yǎng)及動(dòng)物飼養(yǎng)電子書籍
實(shí)驗(yàn)動(dòng)物飼養(yǎng).exe；細(xì)胞培養(yǎng).exe；細(xì)胞培養(yǎng)基.exe

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